Fwd: Re: soil aggregates

From:"Dr. Ian Montgomery"

        Many years ago I sectioned clay specimens semi-thin and ultra-thin. After trials we found that Durcupan was the resin of choice. If you need any more information let me know and I'll try and dig out the necessary data.

Date: Thu, 25 Jul 2002 08:03:56 +0000
From: louise renton <louise_renton@hotmail.com>
Subject: Re: soil aggregates
To: mlm11@cornell.edu, histonet@pathology.swmed.edu, ds278@cornell.edu

I would imagine that some sort of plastic embedding and subsequent sawing/grinding and polishing would be ideal.
Could you perhaps contact a geology or metalurgy laboratory who would advise you on what protocols they would use (unles Dawit is from such a department?!) Failing which, I could send you our protcol used for titanium implants
I once applied for a post in a mining-house lab, and was asked if I realised teh seriousness of mixing up samples - this after 15 years in a medical lab!

BEst regards
From: MaryLou <mlm11@cornell.edu>
To: histonet@pathology.swmed.edu
CC: dawit Solomon <ds278@cornell.edu>
Subject: soil aggregates
Date: Wed, 24 Jul 2002 12:31:48 -0400

Dear Histonetters,

A colleague is wanting to see inside soil aggregates of varying
thicknesses, up to several hundred microns. I was able to make paraffin
sections, 20 microns, by soaking the samples in paraffin for many
hours.  No solvents allowed.  A researcher at NASA gets 1 micron sections
from his dust particles in sulfur. We have no idea how he does it.
Thinner is better. Any suggestions out there? Do any bone grinders have any
ideas? Do you know of anybody else we can ask?
Please include Dawit in your responses. Dawit, do you have anything to add?

Thank you very much.
Mary Lou

Louise Renton
Bone Research Unit
South Africa
Tel & fax +27 11 717 2298
"Time flies like an arrow, friut flies like a banana"

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Dr. Ian Montgomery,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
e-mail: ian.montgomery@bio.gla.ac.uk

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