Fwd: Fw: Mouse testes processing
|From:||"Dr. Ian Montgomery" |
routine but the best fixation and processing I ever had with testes was
using Bouin followed by dioxane dehydration and clearing. Only thing,
what will your safety people think of dioxane.
Dr. Ian Montgomery,
I'm having difficulty with routine processing of mouse testes. I
been able to preserve the intertubular connective tissue. I've
fixative and it's well preserved. It mostly disappears with
paraformaldehyde and 10% neutral buffered formalin. The simple
answer is to
use bouins, but what I'd really like to do is figure out how to preserve
with routine fixation/processing.
My processor is set up as follows:
Prosoft x5 stations
ProPar x3 stations
paraffin x4 stations
I will post a picture to the histonet.org site and will send another
with the path when it's posted.
Also, for those of you using Davidson's fixative on mouse tissues,
there been many problems with subsequent immunostaining?
Thanks for your help,
Managing Director Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri 64110
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Tel: 0141 339 8855
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