Fwd: Fw: Mouse testes processing

From:"Dr. Ian Montgomery"

        Not routine but the best fixation and processing I ever had with testes was using Bouin followed by dioxane dehydration and clearing. Only thing, what will your safety people think of dioxane.

Hello all!

I'm having difficulty with routine processing of mouse testes.  I haven't
been able to preserve the intertubular connective tissue.  I've tried bouins
fixative and it's well preserved.  It mostly disappears with 4%
paraformaldehyde and 10% neutral buffered formalin.  The simple answer is to
use bouins, but what I'd really like to do is figure out how to preserve it
with routine fixation/processing.

My processor is set up as follows:
70% alcohol
Prosoft x5 stations
ProPar x3 stations
paraffin x4 stations

I will post a picture to the histonet.org site and will send another email
with the path when it's posted.

Also, for those of you using Davidson's fixative on mouse tissues, have
there been many problems with subsequent immunostaining?

Thanks for your help,

Teri Johnson
Managing Director Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110

Dr. Ian Montgomery,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
e-mail: ian.montgomery@bio.gla.ac.uk

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