Freezing mouse eyes

From:"Johnson, Teri"

I have been asked to do fresh frozen sectioning on mouse eyes.
Are there any deviations to "normal" flash freezing that I
should know?  I had planned to freeze one as usual (in a
cryomold in OCT) in the Histobath with isopentane, and 
perhaps have the other globe infiltrate for a period of
time in OCT before freezing (2 hours?).

Any suggestions?  Does anybody have any literature references
on this?


Teri Johnson
Managing Director Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110

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