FW: DNA
From: | "Monson, Frederick C." |
> Hi Dolores,
> Extraction of DNA means several things to many of us. Can you be
> more specific about what you are doing the extraction for, and on fixed,
> sectioned or whatever kind of formalin-fixed tissue.
> Also, If you get good results with paraffin embedded specimens, what
> is THAT fixed in?
>
> Please forgive, but I am a little confused.
>
> Thanks,
>
> Fred Monson
>
> Frederick C. Monson, PhD
> Center for Advanced Scientific Imaging
> Schmucker II Science Center
> West Chester University
> South Church Street and Rosedale
> West Chester, Pennsylvania, USA, 19383
> Phone: 610-738-0437
> FAX: 610-738-0437
> fmonson@wcupa.edu
> CASI URL: http://darwin.wcupa.edu/casi/
> WCUPA URL: http://www.wcupa.edu/
> Visitors URL: http://www.wcupa.edu/_visitors/
>
>
> ----------
> From: Dolors Fuster
> Sent: Monday, July 22, 2002 3:26 PM
> To: histonet@pathology.swmed.edu
> Subject: DNA
>
> Hi histonetters !
>
> What do you recommend us to obtain the best results in DNA extraction
> from formalin fixed tissue? should we do any kind of treatment before
> starting the
> extraction? (Water rinses, alcohol changes....)
>
> We are usin Qiagen kit with good results for parafin embeded samples
> fresh tissue and other kind of samples (cell culture, serum...). but
> are not successful with formalin fixed tissue
>
> Thanks in advance
> Dolors Fuster
>
> Tecnic Especialista en
> Anatomia Patologica
> Departament d'Anatomia i Embriologia Humana
> Facultat de Medicina (HCP)
> Universitat de Barcelona
> ICQ 14146798
>
> http://www.fashionmas.com/click/
>
>
>
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