FW: DNA

From:"Monson, Frederick C."


> Hi Dolores,
> 	Extraction of DNA means several things to many of us.  Can you be
> more specific about what you are doing the extraction for, and on fixed,
> sectioned or whatever kind of formalin-fixed tissue.
> 	Also, If you get good results with paraffin embedded specimens, what
> is THAT fixed in?
> 
> 	Please forgive, but I am a little confused.
> 
> Thanks,
> 
> Fred Monson
> 
> Frederick C. Monson, PhD   
> Center for Advanced Scientific Imaging
> Schmucker II Science Center
> West Chester University
> South Church Street and Rosedale
> West Chester, Pennsylvania, USA, 19383
> Phone:  610-738-0437
> FAX:  610-738-0437
> fmonson@wcupa.edu
> CASI URL:  http://darwin.wcupa.edu/casi/
> WCUPA URL:  http://www.wcupa.edu/
> Visitors URL:  http://www.wcupa.edu/_visitors/
> 
> 
> ----------
> From: 	Dolors Fuster
> Sent: 	Monday, July 22, 2002 3:26 PM
> To: 	histonet@pathology.swmed.edu
> Subject: 	DNA
> 
> Hi histonetters !
> 
> What do you recommend us to obtain the best results in DNA extraction 
> from formalin fixed tissue? should we do any kind of treatment before
> starting the 
> extraction? (Water rinses, alcohol changes....)
> 
> We are usin Qiagen kit with good results for parafin embeded samples 
> fresh tissue and other kind of samples (cell culture, serum...). but 
> are not successful with formalin fixed tissue
> 
> Thanks in advance
> Dolors Fuster
> 
> Tecnic Especialista en 
> Anatomia Patologica
> Departament d'Anatomia i Embriologia Humana
> Facultat de Medicina (HCP)
> Universitat de Barcelona
> ICQ 14146798
> 
> http://www.fashionmas.com/click/
> 
> 
> 




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