Dear All
Since I transferred from Research to Hospital Pathology I haven't had much time to read the Histonet. However the recent debate on sterilising instruments stirred my memory, so I thought I would drop you all a line:

When I was in research doing RNA in situ I used to use DMPC ( dimethyl pyrocarbonate) from Sigma instead of DEPC. It appeared to be just as effective when we tested it against RNases.DEPC is quite a hazardous substance whereas DMPC is used in the soft drinks industry and so is probably less hazardous.

From memory it has the Bayer trade name Velcorin and I got the method from an old Boehringer manual.

Mike Bromley

Chief Biomedical Scientist
Pathology
Dumfries & Galloway Royal Infirmary

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