yellow HRP color

From:Cynthia Favara


I just did immunohistochemisty using ascites for  PrP,Prion Protein with an
in house mouse ascites. I used a biotinylated secondary. I got positive
staining, known because we have another in house antibody recognizing the
same protein that was run in parallel.

Positive staining was a yellow-brown with AEC detection. Question is has any
one noticed this color of positive staining before? If yes were you able to
tinker with the IHC and get to a normal red deposition?

Please be gentle after all it is Monday and late in the day!!!

Thanks to all,

Cynthia Favara

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