sections loss after EDTA HIER
Dear
Collegues,
I recently started to try out antigen retrieval with 1mmol EDTA (pH 8.0,
using steaming in a vegetable steamer for 1 hour). Unlike with steaming
with citrate buffer pH 6.0, my sections were completely lost after the
EDTA treatment. We use double-silane coated slides (Superfrost).
Does anybody know why the sections are lost and whether we have to take
another kind of coating?
thanks in advance
jan
-----------------------------------------------------
Jan Bauer, Ph.D
Div. of Neuroimmunology
Brain Research Institute
Spitalgasse 4
A-1090 Vienna
Austria
tel: +43-1-4277 62813
fax: +43-1-4277 9628
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