high contrast lung stain

From:Frank Rosenthal

Does anyone have suggestions for a high contrast (LM) stain for alveolar
boundaries in air-dried (inflated) lung tissue embedded in glycol
methacrylate.   I have tried H&E, Toluidine Blue, Methylene Blue and
Polysciences Multiple Stain with limited success.  The high contrast is
needed for proper processing in an image analysis system.  Any suggestions
or ideas will be very much appreciated.


Frank S. Rosenthal, Ph.D.
Associate Professor
Purdue University School of Health Sciences
1338 Civil Engineering Building
West Lafayette, IN 47907 USA
tel: 765-494-0812, fax: 765-496-1377,
e-mail: frank@purdue.edu

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