coverslipping plastic sections
Deara Trisha,
I do not know by what you mean by "dry"
sections. Like Rachael from Down Under, I do plastics as
well. We coverslip many GMA sections. The slides are air
dried after coming out of the ETOH then placed in 2 changes of xylene prior to
coverslipping.
Depending on the thickness of the mounting
medium bubbbles can form under the coverslip. If the mounting medium is
too thick, very small bubbles may form. If the mounting medium is too
thin, the slides may form bubbles under the coverslip after the mounting medium
has "dried". This is due to shrinkage of the mounting medium.
Another reason bubbles may form when coverslipping
plastic sections is that there may plastic medium sticking up a bit. This
can be scraped off with forceps prior to coverslipping the section.
Good luck Trisha,
Cathy
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