Re: immunostaining

From:louise renton

Could this be due to droplet formation on the tissue section?  Three things 
come to mind: incomplete covering of the section by reagents, drying of the 
reagents on the sections or incomplete dewaxing prior to staining. I would 
imagine that double dipping would cause, if anything, a problem underneath 
the section and not on top of it. You don't say what the patchiness looks 
like.. if it is dark non specific staining then I suppose it could be due to 
"bubbles" in the section trapping reagents beneath the tissue. I have not 
encountered this problem, only one due to poor covering of the section,

Best regards
Louise Renton
Bone Research unit
South Africa

>From: Anissa Choi 
>Subject: immunostaining
>Date: Fri, 05 Jul 2002 00:12:54 -0700
>Lately we have problem of patchy staining with our immuno slides. we
>run the immuno slides using Ventana immunostainer. I was assured by
>Ventana'technical specialist that there is nothing wrong with the machine.
>It was suggested to me that the staining problem may be due to:
>1. Poor quality of the "charged" slides
>2. double dipping of "charged" slides when picking up section---slide
>   is dipped in water and for some reason,gets dry before it is dipped
>   into the water again for picking up section.
>I would think the sections will detach from the slides if there is
>problem with the "charged "slides. I wonder how it can affect the
>Thanks in advance for your opinion.
>Anissa Choi
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