Re: faded DAB


I have not had any success restaining sections, in my experience they either get staining all over or none at all, but if these are precious sections I suppose I would seek the experts advise and give it a try.  Perhaps before restaining all together you could try some of the DAB enhancment methods if they are already staining specifically just very light (faded).

Julie Clark wrote:

> Hi Patsy,
> I know they are DAB because it is the grant protocol, that is why I can't change chromgens.  It seems like I have been using DAB+ since 2000 (when the slides originated), but it doesn't mean they were.  I have been doing immuno for almost 13 years and have never seen anything like this.  Would you try re-staining or do you think you would just get horrible background?
> Thanks,
> Julie
> >>> rueggp  07/10/02 11:21AM >>>
> Julie,
> Are you sure the orginal slides were DAB stained and are now faded?  I have no experience with DAB fading, I have seen other chromogens fad such as AEC.  Another
> thought is that with improved enhanced IHC detection systems maybe we are just not used to looking at the lighter staining we used to get.
> Patsy
> Julie Clark wrote:
> > I have been asked to review some two year-old immuno slides.  Most of the slides are very faded to completely faded.  I have no idea what brand DAB they were using.
> >
> > Has anyone tried to re-stain previously DAB stained immuno slides?  I have to use DAB, I cannot switch chromogens.
> >
> > Is there some way to remove the faded DAB?
> >
> > Thanks,
> >
> > Julie Clark
> > Technical Director
> > Breast Cancer Prevention Center Research Laboratory
> > Room 3021 Lied
> > Univ. of Kansas Med. Center
> > Kansas City, KS
> > Phone: Office: 913-588-3963
> >             Lab:     913-588-3917
> > FAX: 913-588-3821
> > Page: 913-917-6052

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