Re: faded DAB


Are you sure the orginal slides were DAB stained and are now faded?  I have no experience with DAB fading, I have seen other chromogens fad such as AEC.  Another
thought is that with improved enhanced IHC detection systems maybe we are just not used to looking at the lighter staining we used to get.

Julie Clark wrote:

> I have been asked to review some two year-old immuno slides.  Most of the slides are very faded to completely faded.  I have no idea what brand DAB they were using.
> Has anyone tried to re-stain previously DAB stained immuno slides?  I have to use DAB, I cannot switch chromogens.
> Is there some way to remove the faded DAB?
> Thanks,
> Julie Clark
> Technical Director
> Breast Cancer Prevention Center Research Laboratory
> Room 3021 Lied
> Univ. of Kansas Med. Center
> Kansas City, KS
> Phone: Office: 913-588-3963
>             Lab:     913-588-3917
> FAX: 913-588-3821
> Page: 913-917-6052

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