Re: acid maltase
Nancy: I think what you are attempting to do is an Acid
Phophatase Stain, an azo-dye enzymatic reaction. This procedure
will show an increase in staining activity within muscle fibers in a
"measles pattern" in a patient with an acid maltase deficiency.
Substrate used is: Napthol AS-BI Phosphate. We order from
Sigma. And the buffer you are asking about is: 0.2M Acetate
Buffer. Easily made with Sodium Acetate and Glacial Acetic Acid.
Contact me if you would like me to fax you a procedure.
Jean Mitchell, BS, HT (ASCP)
University of Wisconsin Hospital & Clinics
Department of Neurology
> can anyone help me with this "acid maltase" stain ??? i need an 0.2m
> acetate buffer....can i get this pre-made ? if so where ? if i can't
> what type of sodium acetate do i use...trihydrate or anhydrous ???
> also where can i order the sodium alpha naphthyl phosphate from and
> does it go by adifferent name ?? also is acid phosphatase azo-coupling
> technique the same as an acid maltase stain ?? HELP :-/> thanks nancy
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