Re: acid maltase

From:Ronnie_Houston@bshsi.com




     Nancy

     Unless you are intending on using a semi-permeable membrane technique for
     the enzyme histochemical reaction, forget it. The enzyme is extremely
     soluble, and you will lose it all in your incubation media. I believe there
     are commercial suppliers of an antibody to acid maltase, why not try
     immunocytochemistry?

     Acid maltase bears no similarity to acid phosphatase, and the salt you have
     mentioning is not a specific substrate for acid maltase, but for acid
     phosphatase. There are a couple,of hard-to-find substrates that are
     specific for acid maltase, one of which is 2-napthol-alpha-glucoside (can't
     remember the others offhand)

     Suggest you read "Histochemistry" Vol 3, edited by Peter Stoward, or
     "Enzyme Histochemistry: A Laboratory Manual" by Lojda et al (this is
     out-of-print - but you may be able to borrow it through a library) before
     embarking on this.


     Ronnie Houston
     Regional Histology Operations Manager
     Bon Secours Health Partners Laboratories
     5801 Bremo Road
     Richmond, VA 23226


______________________________ Reply Separator _________________________________
Subject: acid maltase
Author:  "Anacko, Nancy L."  at BSHSIBTW
Date:    7/1/02 12:08 PM




       can anyone help me with this "acid maltase" stain ??? i need an
       0.2m acetate
       buffer....can i get this pre-made ? if so where ?
       if i can't what type of sodium acetate do i use...trihydrate or
       anhydrous
       ??? also where can i order the sodium alpha naphthyl phosphate
       from and does
       it go by adifferent name ?? also is acid phosphatase
       azo-coupling technique
       the same as an acid maltase stain ?? HELP :-/> thanks nancy






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