Re: Processing rat brain
The best way is to perfuse the whole animal, remove the brain, and place
it in half sucrose half fix for 2 to 4 hours, then in 30% sucrose for
storage until cryosectioning. I have a very good, detailed procedure if
you want it.
Kathleen Spencer
On Monday, July 15, 2002, at 07:45 AM, Andrew.Shand@north-
bristol.swest.nhs.uk wrote:
> My apologies for raising this old chestnut again but I would like to
> know
> the details of a good schedule for processing rat brain slices. I
> have a
> researcher with a floating section problem rather than the usual brittle
> cutting.
>
> Andy Shand
>
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