Re: Processing rat brain

From:Kathleen Spencer

The best way is to perfuse the whole animal, remove the brain, and place 
it in half sucrose half fix for 2 to 4 hours,  then in 30% sucrose for 
storage until cryosectioning. I have a very good, detailed procedure if 
you want it.

Kathleen Spencer

On Monday, July 15, 2002, at 07:45 AM, Andrew.Shand@north- wrote:

> My apologies for raising this old chestnut again but I would like to 
> know
> the details of a good schedule for processing rat brain slices.  I 
> have a
> researcher with a floating section problem rather than the usual brittle
> cutting.
> Andy Shand

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