Re: Plastic mounting/bubbles

From:Gayle Callis

It also helps to thin the mounting media a bit more!  ARe you talking about
GMA sections?  If so, put a drop of mounting media on edge of slide, just
below GMA embedded section and mount coverslip by lowering it onto drop,
with a tidge of pressure to push the mounting media under coverglass.  

If you are coverslipping PMMA sections with PMMA still present, you will
crack the PMMA with mounting media solvent, giving ugly look to your
sections.   These can be viewed with a coverslip sitting on top of the
section but not permanently mounted.  The coverslip will correct for
refractive index at high power, and you will have nice sections for
photowork, etc.   

At 06:26 PM 7/11/02 -0700, you wrote:
>I have a co-worker who is having difficulty putting cover-slips on his
>"dry" sections embedded in plastic.  (I have never done plastic).  He is
>getting many bubbles.  Please tell me how you avoid them or get them out
>if you do get them. I will pass on your advice.
>U of Washington
Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (lab with voice mail)
406 994-4303 (FAX)


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