Re: Mast cell tryptase

From:Jan Shivers

Dr. Cartun,

Yes, DAKO's anti-human mast cell tryptase DOES stain canine mast cell
tumors.  I use it at 1:50 dilution, 30 minutes incubation in primary, LSAB2
detection kit, with proteinase K pretreatment for 5 minutes.

Jan Shivers
Univ of Minn Veterinary Diagnostic Lab

----- Original Message -----
From: "Richard Cartun" 
Sent: Tuesday, July 09, 2002 3:55 PM
Subject: Mast cell tryptase

> Does anyone know if DAKO's mouse anti-human "Mast cell tryptase" mAb
> stains canine mast cell tumors?  We have a tumor from a dog's leg that
> is strongly CD117 positive and negative for mast cell tryptase, but
> there is no internal control to validate if the antibody cross-reacts.
> Thanks!
> R. Cartun

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