Re: Mast cell tryptase

From:Bryan Hewlett

Richard,

I do not believe that it does.
It does not work in mouse or rat tissues in our hands.
Have you considered checking for the presence of mast cells using Alcian
blue pH 1.0 or the long toluidine blue method?
CD117 reactivity is of course not restricted to mast cells. What is the
immunophenotype of the tumour cells? Are they Histamine positive? The mast
cells of all mammalian species are histamine positive.

Best regards.

Bryan

Bryan R. Hewlett
Technical Specialist
Anatomical Pathology
Hamilton Regional Laboratory Medicine Program
Ontario, Canada


----- Original Message -----
From: "Richard Cartun" 
To: 
Sent: Tuesday, July 09, 2002 5:55 PM
Subject: Mast cell tryptase


> Does anyone know if DAKO's mouse anti-human "Mast cell tryptase" mAb
> stains canine mast cell tumors?  We have a tumor from a dog's leg that
> is strongly CD117 positive and negative for mast cell tryptase, but
> there is no internal control to validate if the antibody cross-reacts.
> Thanks!
>
> R. Cartun
>
>






<< Previous Message | Next Message >>