Re: Mast cells.
Richard and Histonetters,
We routinely stain canine skin lesions for Mast cells using the Quick
Toluidine Blue Method as quoted in "Animal Tissue Techniques" by Gretchen
L. Humason. W H Freeman and Company 1972. This method uses 0.2% Toluidine
Blue in 60% Ethanol.
Tissues are fixed in 4% Phosphate Buffered Formaldehyde and processed
through ethanol and xylene. Sections are taken to ethanol and placed in Tol
Blue for 1 - 2 minutes (longer doesn't make any difference)
We dehydrate quickly in ethanol, clear in xylene and mount in DPX.
Canine Mast Cell granules stain a purple-red colour and stick out like
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