Re: IHC for Glutaraldehyde fixed tissue?
I have had similar problems with glutaraldehyde-fixed tissues. I have had
limited success with a combination of HEIR and trypsin (the staining is not
nearly as strong as with FFPE tissues).
"Miller, Janet" wrote:
> Hello. I have some old cat brain tissue that was fixed in 2% Glutaraldehyde
> and 2% paraformeldehyde in PBS, then paraffin embedded. As one would
> expect, we are having trouble trying to do any immunohistochemistry on the
> tissue, cut in 8 um sections. We are currently attempting to do GFAP
> staining without success. I have tried to 'break apart' the glutaraldehyde
> bonds with a 1% Triton X solution for 10 min. This seemed to help a little
> bit, but it is still not sufficient. I was wondering if anyone out there
> has had any luck with staining tissue of this nature, or is it a lost cause?
> Any suggestions would be appreciated! Thank you very much!
> Janet Miller
> Department of Neurosurgery
> Wayne State University School of Medicine
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