Re: IHC for Glutaraldehyde fixed tissue?

From:Kimberly L Merriam


I have had similar problems with glutaraldehyde-fixed tissues.  I have had
limited success with a combination of HEIR and trypsin (the staining is not
nearly as strong as with FFPE tissues).

Good luck,
Kim Merriam
Cambridge, MA

"Miller, Janet" wrote:

> Hello.  I have some old cat brain tissue that was fixed in 2% Glutaraldehyde
> and 2% paraformeldehyde in PBS, then paraffin embedded.  As one would
> expect, we are having trouble trying to do any immunohistochemistry on the
> tissue, cut in 8 um sections.  We are currently attempting to do GFAP
> staining without success.  I have tried to 'break apart' the glutaraldehyde
> bonds with a 1% Triton X solution for 10 min.  This seemed to help a little
> bit, but it is still not sufficient.  I was wondering if anyone out there
> has had any luck with staining tissue of this nature, or is it a lost cause?
> Any suggestions would be appreciated!  Thank you very much!
> Sincerely,
> Janet Miller
> Department of Neurosurgery
> Wayne State University School of Medicine

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