Re: Escherichia coli
In my experience, E. coli will label beautifully in formalin-fixed,
paraffin-embedded. Like all IHC stains, the majority of your success
will depend on the quality of your primary antibody.
>>> =?UNKNOWN?Q?Agust=EDn?= Venzano
07/12/02 09:38AM >>>
Dear netters: I need contacting any of you with current or past
on paraffin IHC (i.e. Streptavidin-biotin or ABC IHC) aimed at the
identification of VT + (=Verotoxigenic ) strains of Escherichia coli
jejunal or ileal sections. I'm specially interested in listening to
advise of those that have used primary antisera to Esherichia coli
or "O" antigens, specially "O 111" to mark such bacteria when attached
the epitelial apical border.
I'd be also interested in getting the exact processing protocol that
been used in these cases.
Thank you in advance
Agustin Jose Venzano Halliburton
DVM-INTA Castelar, Argentina
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