Re: Collagen type II


I used to use hyaluronidase and chondroitinase ABC for collagen II from
U of Iowa, but it was very complicated and expensive, so we switched to
premade pepsin from Research Genetics with good results and it is a lot
easier and cheaper.  If your poor results include non-specific
background staining, I can share some blocking ideas with you.

Anne Undersander wrote:

> Histonet, Could somebody please share their protocol for IHC collagen
> type II staining?  The antibodies are from the U of Iowa Hybridoma
> Bank and will be used on monkey bones.  The initial staining run
> produced poor results.  Is there a better antigen retrieval method
> then using hyaluronidase? Thanks.Anne Undersander HT(ASCP)
> Vet. Med./Animal Science
> University of Minnesota

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