CD003;  
clone: PS1 (mouse IgG2a)

• Mfgr/Vendor:  Novocastra, #NCL-CD3-PS1
• Control:  Tonsil, lymph node
• Pretreatment:  10mM Citrate solution inside a microwave pressure cooker and cooked at pressure for 15 minutes followed by a 10 minute cooldown.   Wash in buffer.   
• Dilution:  Apply at 1:25 and incubate for 40 minutes @RT.
• Secondary:  NA
• Detection:  Apply EnVision+TM , DAKO Kit # K4007 (Mouse) and incubate 30 minutes @RT.  Wash as above
• Chromogen:  DAB
  

Hope this helps. If you need any further info, email me.

Patti Loykasek
Phenopath Laboratories
Seattle, WA


From: Amos Brooks <amosbrooks@comcast.net>
Date: Wed, 10 Jul 2002 19:30:22 -0400
To: Histonet <histonet@pathology.swmed.edu>
Subject: CD3 Polyclonal

Hi,
   What's up with the CD3 polyclonal antibody? I heard that most companies are going to end up being back ordered because a rabbit died. We have not been having as good results in our trials of the monoclonal CD3. Is anyone using it with as good results as the polyclonal?
Amos Brooks