Hi Cynthia

Yellow-brown is indicative of over-staining with AEC.  Dilute your primary and/or shorten incubation time and you should be good to go!

Noelle

-----Original Message-----
From: Cynthia Favara [mailto:cfavara@niaid.nih.gov]
Sent: Monday, July 01, 2002 4:00 PM
To: HistoNet Server (E-mail)
Subject: yellow HRP color


All,

I just did immunohistochemisty using ascites for  PrP,Prion Protein with an
in house mouse ascites. I used a biotinylated secondary. I got positive
staining, known because we have another in house antibody recognizing the
same protein that was run in parallel.

Positive staining was a yellow-brown with AEC detection. Question is has any
one noticed this color of positive staining before? If yes were you able to
tinker with the IHC and get to a normal red deposition?

Please be gentle after all it is Monday and late in the day!!!

Thanks to all,

Cynthia Favara