RE: faded DAB
Try popping the coverslips, running them back to water and then placing them
in a 10% Cupric Chloride (& DI water) solution for ten minutes. This may
enhance your DAB staining.
From: rueggp [mailto:email@example.com]
Sent: Thursday, July 11, 2002 3:12 PM
To: Julie Clark; HistoNet Server
Subject: Re: faded DAB
I have not had any success restaining sections, in my experience they either
get staining all over or none at all, but if these are precious sections I
suppose I would seek the experts advise and give it a try. Perhaps before
restaining all together you could try some of the DAB enhancment methods if
they are already staining specifically just very light (faded).
Julie Clark wrote:
> Hi Patsy,
> I know they are DAB because it is the grant protocol, that is why I can't
change chromgens. It seems like I have been using DAB+ since 2000 (when the
slides originated), but it doesn't mean they were. I have been doing immuno
for almost 13 years and have never seen anything like this. Would you try
re-staining or do you think you would just get horrible background?
> >>> rueggp 07/10/02 11:21AM >>>
> Are you sure the orginal slides were DAB stained and are now faded? I
have no experience with DAB fading, I have seen other chromogens fad such as
> thought is that with improved enhanced IHC detection systems maybe we are
just not used to looking at the lighter staining we used to get.
> Julie Clark wrote:
> > I have been asked to review some two year-old immuno slides. Most of
the slides are very faded to completely faded. I have no idea what brand
DAB they were using.
> > Has anyone tried to re-stain previously DAB stained immuno slides? I
have to use DAB, I cannot switch chromogens.
> > Is there some way to remove the faded DAB?
> > Thanks,
> > Julie Clark
> > Technical Director
> > Breast Cancer Prevention Center Research Laboratory
> > Room 3021 Lied
> > Univ. of Kansas Med. Center
> > Kansas City, KS
> > Phone: Office: 913-588-3963
> > Lab: 913-588-3917
> > FAX: 913-588-3821
> > Page: 913-917-6052
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