Fred,
I've never tried Nickel, though I have had experience with Copper but only in double labelling studies (Brown DAB v blue DAB or Blue DAB v Red alkaline phosphatase (fuschine complex))

Sorry,

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html

-----Original Message-----
From: Monson, Frederick C. [mailto:fmonson@wcupa.edu]
Sent: Thursday, 11 July 2002 9:49
To: 'Tony Henwood'
Cc: 'List-HistoPath'
Subject: RE: faded DAB

Tony,
        If the DAB is still where it ought to be, would it be possible to
try the Nickel again?  Or for the first time?

Just wondering,

Fred Monson

> ----------
> From:         Tony Henwood
> Sent:         Wednesday, July 10, 2002 6:59 PM
> To:   'Julie Clark'; HistoNet@pathology.swmed.edu
> Subject:      RE: faded DAB
>
> Julie,
> I doubt whether it would be the brand of DAB. Dyes in slides will
> eventually fade over time. What we can do is keep the slides away from
> bright light (ie sunlight) and look at the mountant we are using. Some
> mountants retard fading better than others. In my experience this is often
> the major cause of early fading of stained slides in my opinion.
>
> To bring back the brown colour, you could try osmicating decoverslipped
> sections (Beware osmium tetroxide is dangerous stuff). This has worked for
> me about 3 of 10 times I have tried it.
>
> Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
> Laboratory Manager
> The Children's Hospital at  Westmead,
> Locked Bag 4001, Westmead, 2145, AUSTRALIA.
> Tel: (02) 9845 3306
> Fax: (02) 9845 3318
>
> http://www.histosearch.com/homepages/TonyHenwood/default.html
> http://us.geocities.com/tonyhenwoodau/index.html
>
>
> -----Original Message-----
> From: Julie Clark [ mailto:jclark@kumc.edu]
> Sent: Wednesday, 10 July 2002 22:55
> To: HistoNet@pathology.swmed.edu
> Subject: faded DAB
>
>
> I have been asked to review some two year-old immuno slides.  Most of the
> slides are very faded to completely faded.  I have no idea what brand DAB
> they were using. 
>
> Has anyone tried to re-stain previously DAB stained immuno slides?  I have
> to use DAB, I cannot switch chromogens.
>
> Is there some way to remove the faded DAB?
>
> Thanks,
>
> Julie Clark
> Technical Director
> Breast Cancer Prevention Center Research Laboratory
> Room 3021 Lied
> Univ. of Kansas Med. Center
> Kansas City, KS
> Phone: Office: 913-588-3963
>             Lab:     913-588-3917
> FAX: 913-588-3821
> Page: 913-917-6052
>
>
>
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