Morning Anissa,
	The microtome will need to be dried before restarting.  Condensation
forms after the refrigeration is turned off.

	You might want to consult the following for more information about
formaldehyde and/or glutaraldehyde disinfection.
		http://www.nda.ox.ac.uk/wfsa/html/u07/u07_015.htm#acti

		http://ehs.ucdavis.edu/sftynet/sn-51.html

		http://www.cdc.gov/ncidod/hip/dsguide/dsguide.pdf [CDC Draft
guideline] (not very helpful)

		http://occ-env-med.mc.duke.edu/oem/tb-req.htm [CDC Request
for Comments]

		Cheney, J. E. et al. 1995. Formaldehyde disinfection in
laboratories: limitations and hazards. Br. J. Biomed. Sci. 52(3):195-201.

	My recommendation is to ask Leica for evidence of the efficacy of
their product.  If it is stated to be satisfactorily effective and has been
registered with the FDA and/or OSHA and/or CDC, then you should be on solid
ground.  

	The issues surrounding formaldehyde and glutaraldehyde are still in
some flux for the latter but pretty much securely set for the former.

Hope this helps,

Fred Monson

Frederick C. Monson, PhD   
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone:  610-738-0437
FAX:  610-738-0437
fmonson@wcupa.edu
CASI URL:  http://darwin.wcupa.edu/casi/
WCUPA URL:  http://www.wcupa.edu/
Visitors URL:  http://www.wcupa.edu/_visitors/


> ----------
> From: 	Anissa Choi
> Sent: 	Friday, July 5, 2002 2:31 AM
> To: 	histonet@pathology.swmed.edu
> Subject: 	disinfection of Leica Cryostat
> 
> 
> We just got a new Leica Cryostat. The company recommend spray disinfection
> 
> with Leica Cryofect. However, our pathologist is not
> convinced that it is effective against TB. We decide to use formaldehyde
> vapour by placing 30ml of 37% formaldehyde in the chamber overnight(with
> the 
> Cryostat switched off and lid closed ) I would like to know if this 
> procedure really does the work. Also, after the disinfection,
> do we really need to remove the microtome to dry thoroughly before we
> put it back in and turn the machine on?
> 
> Thanks in advance for your input !
> 
> 
> Anissa Choi
> 
> 
> 
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