RE: disinfection of Leica Cryostat

From:"Weems, Joyce"

We do not remove the microtome. After the formaldehyde, we flush with 1)
bleach water, 2) many flushes of clean water, and finish off with 3) a good
flush of absolute alcohol. Then dry with a hair dryer (secured by closing
the lid on it), leave it open from Sat to Mon morning before turning it on.
If it's not thoroughly dry it will freeze and not be usable. 

Leica Cryofect is not available yet in the US (that I am aware of). The
above method works and is cheaper, I'm sure!

Good luck!

Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta
404-851-7831 - fax

 -----Original Message-----
From: 	Anissa Choi [] 
Sent:	Friday, July 05, 2002 2:31 AM
Subject:	disinfection of Leica Cryostat

We just got a new Leica Cryostat. The company recommend spray disinfection 
with Leica Cryofect. However, our pathologist is not
convinced that it is effective against TB. We decide to use formaldehyde
vapour by placing 30ml of 37% formaldehyde in the chamber overnight(with the

Cryostat switched off and lid closed ) I would like to know if this 
procedure really does the work. Also, after the disinfection,
do we really need to remove the microtome to dry thoroughly before we
put it back in and turn the machine on?

Thanks in advance for your input !

Anissa Choi

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