RE: Processing rat brain

Spent many an unhappy hour doing that.  Human cerebellum and foetal brain
being remebered with particular horror!  Though I would be only to happy to
teach the technique - I suspect the researcher would not be happy to learn.
If she has any sense that is.


-----Original Message-----
From: Smith, Allen []
Sent: 15 July 2002 15:16
Subject: RE: Processing rat brain

Although it is time-consuming, good sections that float off can be processed
manually, using a bent glass rod.  (Heat the tip of stirring rod and jam it
against a heat-resistant table top to make a bent rod.) 

Allen A. Smith, Ph.D.
Barry University
School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161-6695 

-----Original Message-----
Sent: Monday, July 15, 2002 8:46 AM
Subject: Processing rat brain

My apologies for raising this old chestnut again but I would like to know
the details of a good schedule for processing rat brain slices.  I have a
researcher with a floating section problem rather than the usual brittle

Andy Shand

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