Not sure what you means by a floating section problem. I work with lots a
rodent brain tissue and think that adequate fixation is my biggest
nightmare. If the specimen is adequately fixed processing can vary depending
on your processor, number of stations etc. Problems I run into are when
investigators want to fix whole brain for 4 hours or less. Just does not do
the tissue justice. Try lowering temp of water bath, I usually float brain
sections on a bath about 3-5C cooler than for other tissues that have been
similarly processed.

Cynthia Favara

-----Original Message-----
From: Andrew.Shand@north-bristol.swest.nhs.uk
[mailto:Andrew.Shand@north-bristol.swest.nhs.uk]
Sent: Monday, July 15, 2002 6:46 AM
To: histonet@pathology.swmed.edu
Subject: Processing rat brain


My apologies for raising this old chestnut again but I would like to know
the details of a good schedule for processing rat brain slices.  I have a
researcher with a floating section problem rather than the usual brittle
cutting.

Andy Shand