RE: Plastic mounting/bubbles

From:Rachel Peat

RE: Plastic mounting/bubbles

Dear Trisha,

I work with bone embedded in methyl methacrylate resin (MMA or p-MMA) and I'm not sure what you'd need 'dry' sections for.  If they just want to clear the section of the surrounding resin for something like analysis of fluorescent label in bone, then they could follow this protocol:

1.  Sit slides in 100% acetone for 20 mins
2.  Rinse with 100% ethanol twice
3.  Rinse with 100% xylene
4.  Coverslip with xylene based mounting media, (in our case Eukitt).

Because we use these cleared slides for fluorescent analysis, we make sure that our solutions are very fresh/clean and free of anything like Eosin which can interfere with the label.

I hope this helps, and if you have any other questions, please don't hesitate to ask.



Rachel Peat (BScHons)
Orthopaedic Research Unit
Children's Hospital at Westmead
Westmead, NSW 2145,
Telephone: +61 2 9845 2949
Facsimile: +61 2 9845 3078

-----Original Message-----
From: P. Emry []
Sent: Friday, 12 July 2002 11:26
Subject: Plastic mounting/bubbles


I have a co-worker who is having difficulty putting cover-slips on his
"dry" sections embedded in plastic.  (I have never done plastic).  He is
getting many bubbles.  Please tell me how you avoid them or get them out
if you do get them. I will pass on your advice.


U of Washington

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