RE: Need help from someone with a Shandon Pathcentre that does de rm work
I'd switch from sponges to end papers. We get them at the local beauty
supply store. Give it a try!
Respectfully,
Bruce Gapinski HT(ASCP)
-----Original Message-----
From: Weems, Joyce [mailto:JWEEMS@sjha.org]
Sent: Wednesday, July 03, 2002 9:21 AM
To: 'Jennings-Siena, Debbie';
histonet@pathology.swmed.edu
Subject: RE: Need help from someone with a Shandon
Pathcentre that does de rm work
Are you moistening your sponges in formalin before use using
vacuum on the
processor?
Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta
404-851-7376
404-851-7831 - fax
-----Original Message-----
From: Jennings-Siena, Debbie
[mailto:DebbieSi@BaylorHealth.edu]
Sent: Wednesday, July 03, 2002 12:08 PM
To: histonet@pathology.swmed.edu
Subject: Need help from someone with a Shandon
Pathcentre that does
derm work
I am needing help from anyone that does primarily
dermatology specimens and
who uses a Shandon Pathcentre Processor. I have had a
Pathcentre since
January and am having some difficulties in processing the
specimens. I
would like to compare my processing schedule to others if
you would please
share, also any thoughts on sponges, and biopsy cassettes.
I only put small
skins on this process, I use a longer program for ellipses
and tissues that
are thicker than 4 mm in thickness and punches 4 mm and up.
The problem
that I am having is that shaves and small pieces of tissue
are shriveled up
and it will not be all the tissues, only 1 out of 2 pieces
(bisected) or 1
or 2 out of 3 pieces. The microscopic sections are terrible
and unreadable.
I have tried reprocessing but it does not help. We are
currently using
sponges, thinking that our tissues are bunching in the
corners of the
cassette and not getting processed well. The sponges help
but are terrible
for the carryover and are not helping the bottom line any.
I would love to
stop using the sponges but need the tissues to be processed
well also. The
problem is also most profound on Monday after the weekend.
So, I am sure
that they are fixed well. I could gladly send slides for
anyone to help me.
I have been around for quite a long time, but this problem
is stumping me
and I am losing a lot of hair. I would appreciate any input
that someone
would give me. Thanks a lot. I hope I can repay the favor.
Debbie J. Siena, HT(ASCP)QIHC
Histopathology Manager
Baylor University Medical Center
3500 Gaston Ave.
Dallas, TX 75246
214-820-2465 vm
214-820-4110 fax
debbiesi@baylordallas.edu
My processing schedule is:
10% NB Formalin 30 min ambient
vacuum/pressure
Tidal/Stir
10% NB Formalin 45 min ambient
vacuum/pressure
Tidal/Stir
70% Alcohol 18 min ambient
vacuum/pressure
Tidal/Stir
80% Alcohol 18 min ambient
vacuum/pressure
Tidal/Stir
95% Alcohol 18 min ambient
vacuum/pressure
Tidal/Stir
100% alcohol 18 min ambient
vacuum/pressure
Tidal/Stir
100% Alcohol 18 min ambient
vacuum/pressure
Tidal/Stir
100% Alcohol 18 min ambient
vacuum/pressure
Tidal/Stir
Xylene 18 min ambient
vacuum/pressure
Tidal/Stir
Xylene 18 min ambient
vacuum/pressure
Tidal/Stir
Paraffin 18 min 60C
vacuum/pressure Tidal/Stir
Paraffin 18 min 60C
vacuum/pressure Tidal/Stir
Paraffin 18 min 60C
vacuum/pressure Tidal/Stir
Paraffin 18 min 60C
Vacuum/pressure Tidal/Stir
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