RE: Need help from someone with a Shandon Pathcentre that does de rm work

From:Bruce Gapinski

I'd switch from sponges to end papers. We get them at the local beauty
supply store. Give it a try!
Respectfully,
	Bruce Gapinski	HT(ASCP)

		-----Original Message-----
		From:	Weems, Joyce [mailto:JWEEMS@sjha.org]
		Sent:	Wednesday, July 03, 2002 9:21 AM
		To:	'Jennings-Siena, Debbie';
histonet@pathology.swmed.edu
		Subject:	RE: Need help from someone with a Shandon
Pathcentre that does de	rm work

		Are you moistening your sponges in formalin before use using
vacuum on the
		processor?


		Joyce Weems
		Pathology Manager
		Saint Joseph's Hospital of Atlanta
		404-851-7376
		404-851-7831 - fax

		 -----Original Message-----
		From: 	Jennings-Siena, Debbie
[mailto:DebbieSi@BaylorHealth.edu] 
		Sent:	Wednesday, July 03, 2002 12:08 PM
		To:	histonet@pathology.swmed.edu
		Subject:	Need help from someone with a Shandon
Pathcentre that does
		derm work


		I am needing help from anyone that does primarily
dermatology specimens and
		who uses a Shandon Pathcentre Processor.  I have had a
Pathcentre since
		January and am having some difficulties in processing the
specimens.  I
		would like to compare my processing schedule to others if
you would please
		share, also any thoughts on sponges, and biopsy cassettes.
I only put small
		skins on this process, I use a longer program for ellipses
and tissues that
		are thicker than 4 mm in thickness and punches 4 mm and up.
The problem
		that I am having is that shaves and small pieces of tissue
are shriveled up
		and it will not be all the tissues, only 1 out of 2 pieces
(bisected) or 1
		or 2 out of 3 pieces.  The microscopic sections are terrible
and unreadable.
		I have tried reprocessing but it does not help.  We are
currently using
		sponges, thinking that our tissues are bunching in the
corners of the
		cassette and not getting processed well.  The sponges help
but are terrible
		for the carryover and are not helping the bottom line any.
I would love to
		stop using the sponges but need the tissues to be processed
well also.  The
		problem is also most profound on Monday after the weekend.
So, I am sure
		that they are fixed well.  I could gladly send slides for
anyone to help me.
		I have been around for quite a long time, but this problem
is stumping me
		and I am losing a lot of hair.  I would appreciate any input
that someone
		would give me. Thanks a lot. I hope I can repay the favor.
		Debbie J. Siena, HT(ASCP)QIHC
		Histopathology Manager
		Baylor University Medical Center
		3500 Gaston Ave.
		Dallas, TX 75246
		214-820-2465 vm
		214-820-4110 fax
		debbiesi@baylordallas.edu


		My processing schedule is:

		10% NB Formalin	30 min		ambient
vacuum/pressure
		Tidal/Stir
		10% NB Formalin	45 min		ambient
vacuum/pressure
		Tidal/Stir
		70% Alcohol		18 min		ambient
vacuum/pressure
		Tidal/Stir
		80% Alcohol		18 min		ambient
vacuum/pressure
		Tidal/Stir
		95% Alcohol		18 min		ambient
vacuum/pressure
		Tidal/Stir
		100% alcohol		18 min		ambient
vacuum/pressure
		Tidal/Stir
		100% Alcohol		18 min		ambient
vacuum/pressure
		Tidal/Stir
		100% Alcohol		18 min		ambient
vacuum/pressure
		Tidal/Stir
		Xylene			18 min		ambient
vacuum/pressure
		Tidal/Stir
		Xylene			18 min		ambient
vacuum/pressure
		Tidal/Stir
		Paraffin			18 min		60C
		vacuum/pressure		Tidal/Stir
		Paraffin			18 min		60C
		vacuum/pressure		Tidal/Stir
		Paraffin			18 min		60C
		vacuum/pressure		Tidal/Stir
		Paraffin			18 min		60C
		Vacuum/pressure		Tidal/Stir








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