Mast cell staining

From:Cheryl Crowder

Richard - We do a lot of staining for mast cells.  We have found that a 
modified Giemsa (with Jenner staining) and the Toluidine Blue are 
excellent for determining both types of mast cells.  In addition to the 
Alcian Blue at pH 1, you can try the Thionine pH 1.  That stain will 
stain only mast cells, so you need to make your tissue area first to be 
able to tell the tissue orientation when the stain is complete.  In most 
cases the mast cell tumor is diagnosed not only on number of mast cells 
but the pattern in which the tissues are.  Hope this helps.  Cheryl

Cheryl Crowder, BA, HTL(ASCP)
Chief Technologist
Department of Pathobiological Sciences
School of Veterinary Medicine
Louisiana State University
Baton Rouge, LA  70803

(225) 578-9784
FAX  (225) 578-9720

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