re:manual immuno staining with Ventana products

From:Schmang155@aol.com


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That is a really good question. Print your protocols for each stain and 
antibody and see if heat is employed in any of the steps. I would think that 
you could use the reagents following the time used on the printed protocols. 
Of course you would have to document the amount of reagent drops you used 
because the machine will not have that amount recorded as it does 
automatically when you do the test by machine.  I have counterstained this 
way--when the machine gliched at the end of a run and it worked out nicely.  
Your Her2 neu are another thing---they may have to be done by machine only 
since they are so touchy---Hope this helps, Call me anytime if I can help. We 
have all the Ventana stainers and love them!
                Angie Schmidt  Tri- Health  Cincinnati---513--872-1508

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<HTML><FONT FACE=arial,helvetica><FONT  COLOR="#000080" SIZE=2 FAMILY="SCRIPT" FACE="Comic Sans MS" LANG="0">That is a really good question. Print your protocols for each stain and 
<BR>antibody and see if heat is employed in any of the steps. I would think that 
<BR>you could use the reagents following the time used on the printed protocols. 
<BR>Of course you would have to document the amount of reagent drops you used 
<BR>because the machine will not have that amount recorded as it does 
<BR>automatically when you do the test by machine.  I have counterstained this 
<BR>way--when the machine gliched at the end of a run and it worked out nicely.  
<BR>Your Her2 neu are another thing---they may have to be done by machine only 
<BR>since they are so touchy---Hope this helps, Call me anytime if I can help. We 
<BR>have all the Ventana stainers and love them!
<BR>                Angie Schmidt  Tri- Health  Cincinnati---513--872-1508</FONT><

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