"staining" for copper
|From:||Keith Ryan <firstname.lastname@example.org>|
Can anyone comment on rubeanic acid and pyroantimonate methods for copper?
There is a visitor to our lab who brought specimens treated with these methods wanting to confirm aspects of exposure/accumulation by electron microscopy with x-ray microanlysis. Normally I would use freeze-drying but cryomethods were not possible in her home university.
She has pictures from semithin/Toluidine Blue sections showing granules, unstained sections from pyroantimonate and rubeanic acid methods which show dense bodies by light microscopy but not a lot in the TEM. Stained granules could be anything that is not electron-dense but ........
Any comments would be welcome!
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