"staining" for copper

From:Keith Ryan <kpr@mba.ac.uk>

Hello Histonetters

Can anyone comment on rubeanic acid and pyroantimonate methods for copper?

There is a visitor to our lab who brought specimens treated with these methods wanting to confirm aspects of exposure/accumulation by electron microscopy with x-ray microanlysis. Normally I would use freeze-drying but cryomethods were not possible in her home university.

She has pictures from semithin/Toluidine Blue sections showing granules, unstained sections from pyroantimonate and rubeanic acid methods which show dense bodies by light microscopy but not a lot in the TEM. Stained granules could be anything that is not electron-dense but ........

Any comments would be welcome!

Keith Ryan
Marine Biological

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