(no subject)


Lately in the lab that I work in the pathologists have complained of "bubbly" 
or "vacuated" nuclei of the epithelial cells in the glands.  The rest of the 
tissue appears to be normal.  One theory they've brought to us is the use of 
freeze spray when sectioning is leaving ice crystals behind and creating this 
artifact.  Any input/suggestions or information on similar experiences would 
be greatly appreciated.   Thank you in advance.

Jennifer Macias, HT(ASCP) QIHC

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