limits of torture (to tissues in IHC)

From:Sunil Thomas K


 This to do with double or triple IHC labelling
studies. For eg If one has to detect BrdU together
with a cytoplasmic protein. BrdU incorporated in the
DNA will be visible only after some DNA denaturation
(1-4NHCl, or DNAase or pepsin etc). Wouldn't this
destroy the structure of the protein that one is
trying to detect. Is there any solution. 

Sunil Thomas K

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