|From:||Vicki Kalscheur <firstname.lastname@example.org>|
I am a graduate student working on my MS in the Comparative Orthopedic
Research Lab at the UW-Madison.
My project involves examining chondrocyte viability in bovine patella
samples over time. Part of my project is examining the articular
cartilage for apoptotic chondrocytes. I will be using Anti-Caspace 3,
Anti-PARP, and Tunel assays to do this.
I am, however, having a problem regarding this procedure. It seems
that my samples will not adhere to the slides during the assays.
After about 4 washes (only 1/4 of the way through each assay) the
sections fall off the slides and float in the solution.
I have tried Aminoalkylsilane coated slides, and a product called HALT
to help adhere the sections but have had no luck. I am currently
using EDTA to decalcify the sections and frozen sectioning. I have
considered using paraffin embedded samples to secure my samples more
effectively but am afraid of the damage to the chondrocytes during
heating. We also plan on trying some free-floating analysis.
I would greatly appreciate any suggestions anyone could provide. My
email is email@example.com. My fax is 608-263-7930 (please put
attention Beth at the top)
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