Von Willebrand Factor staining
|From:||Garry Ashton <GAshton@picr.man.ac.uk>|
As some of you may be aware from previous e-mails I am currently
immunostaining FFPE sections to identify blood vessels.
Does anybody have a good reason why on a protocol I have been shown, the
sections are treated with 0.2% proteinase (protease I think) for 8 mins at
37 degrees, immediately followed after washing by the more conventional Ag
retrieval method using trypsin.
Any help and advice gratefully received.
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