Von Willebrand Factor staining

From:Garry Ashton <GAshton@picr.man.ac.uk>

Dear all,
As some of you may be aware from previous e-mails I am currently
immunostaining FFPE sections to identify blood vessels.
Does anybody have a good reason why on a protocol I have been shown,  the
sections are treated with 0.2% proteinase (protease I think) for 8 mins at
37 degrees, immediately followed  after washing by the more conventional Ag
retrieval method using trypsin.
Any help and advice gratefully received.

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