Thant sounds like a button motto! RE: Getting the Eosin out sol ution

From:"Morken, Tim" <tim9@cdc.gov>

Patsy wrote:

"With HISTONET all you have to do is ask and you will receive"

That could go on a Histonet button for NSH!

Tim Morken
Atlanta

-----Original Message-----
From: Patsy.Ruegg@UCHSC.edu [mailto:Patsy.Ruegg@UCHSC.edu]
Sent: Thursday, July 19, 2001 12:22 PM
To: histonet@pathology.swmed.edu
Subject: Getting the Eosin out solution


 
-----Original Message-----
From: Ruegg Patsy 
Sent: Thursday, July 19, 2001 10:21 AM
To: 'KOKOPELLI_2'; Ruegg Patsy
Subject: Getting the Eosin out solution


Thanks to all your advise.
What I did worked like a charm.
I put a few drops of strong (10 M) sodium hydroxide in 150 mls of DI water
and dipped the eosin counterstained DAB reacted slides in this solution, 2
dips was all it took and all the eosin came out without affecting the DAB at
all, it made the hematoxylin stained nuclei nice and blue also.
With HISTONET all you have to do is ask and you will receive.
Patsy Ruegg

-----Original Message-----
From: KOKOPELLI_2 [mailto:KOKOPELLI_2@email.msn.com]
Sent: Tuesday, July 17, 2001 9:13 PM
To: Patsy.Ruegg@UCHSC.edu
Subject: Getting the Eosin out 


Patsy
 
You can put the slides into some ammonia H20 like you would use to blue your
slides in for an H&E stain. The eosin is a acidic dye and the ammonia being
basic will wash out the eosin. Through some trial and error tinkering with
stains I learned this lesson. 
 
I don't know what effect this will have on the DAB? but I would guess very
little, since we sometimes put varied acidic & basic reagents on our immuno
slides for counterstaining purposes for the Docs'.
 
If you try this and it works out, with good results please let me know. I am
always interested in truoble shooting techniques.
 
Brent A. Norris H.T.(ASCP)
Technical Supervisor
Special Procedures Laboratory
University of Texas Medical Branc

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