2 Drops of Buffer, 4 drops of DAB, then 2 drops of H202.  2 drops more for
the nickel(optional)


                                                                                 
                    Elizaha@aol.c                                                
                    om                   To:     histonet@pathology.swmed.edu    
                                         cc:                                     
                    07/23/01             Subject:     vector DAB kit             
                    08:19 AM                                                     
                                                                                 
                                                                                 




   Hi,

I have lost the protocol for mixing the Vector kit for Dab. I was wondering

if someone could refresh my memory (such as it is).

I think it was 5 ml H20, add three drops buffer, 1 drop DAB, then 1 drop
H2O2.
And I can't remember at all how much of the ni