Re: how to remove eosin

From:Amos Brooks <>

    It's difficult to remove DAB (even intentionally), so don't worry about
loosing the chromogen. The easiest way to remove the eosin is with what ever
you are using as a bluing reagent. Run the slides back to water (much of the
eosin will be removed here) then leave the slides in the bluing (ammonia
water usually) for about 5 minuets. Then run the slides back to xylene.
no sweat
Amos Brooks

----- Original Message -----
From: <>
To: <>
Sent: Tuesday, July 17, 2001 11:05 AM
Subject: how to remove eosin

> We made a mistake and counter stained DAB developed IHC slides.  How can
> remove the eosi

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