try an alkaline solution like the bluing agent (i.e. lithium carbonate) used for the H&E. this will remove eosin while leaving hematoxylin staining intact.

Vinnie


Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, SC 29425
Ph: 843-792-6353
fax: 843-792-8974

>>> <Patsy.Ruegg@UCHSC.edu> 07/17/01 11:05AM >>>
We made a mistake and counter stained DAB developed IHC slides.  How can we
remove the eos