Re: Von Willebrand Factor staining

From:"Jan Shivers" <>

No clue.  I only use Proteinase K (DAKO) for 5 minutes at room temperature,
followed by a 10 minute tap water rinse, and the antibody works beautifully.
How can one beat the ease of tissue enzyme digestion for only 5 minutes at
room temperature, anyway?

Jan Shivers
Univ. of Minnesota

----- Original Message -----
From: "Garry Ashton" <>
To: "'histonet'" <>
Sent: Monday, July 16, 2001 9:00 AM
Subject: Von Willebrand Factor staining

> Dear all,
> As some of you may be aware from previous e-mails I am currently
> immunostaining FFPE sections to identify blood vessels.
> Does anybody have a good reason why on a protocol I have been shown,  the
> sections are treated with 0.2% proteinase (protease I think) for 8 mins at
> 37 degrees, immediately followed  after washing by the more conventional
> retrieval method using trypsin.
> Any help and advice gratefully rece

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