Re: Von Willebrand Factor staining

From:Barbara Wright <>


The only  reason I can see to use two enzymatic antigen retrievals is that the
trypsin antigen retrieval in the protocol is not sufficient enough.  I actually
use DAKO's  vonWillebrand Factor VIII at a dilution of 1:4000 using DAKO
trypsin  for 30 minutes in 37C water bath for my antigen retrieval. Let me know
if you want me to send you my entire protocol.


Gary Asthenia wrote:

> Dear all,
> As some of you may be aware from previous e-mails I am currently
> immunostaining FFPE sections to identify blood vessels.
> Does anybody have a good reason why on a protocol I have been shown,  the
> sections are treated with 0.2% proteinase (protease I think) for 8 mins at
> 37 degrees, immediately followed  after washing by the more conventional Ag
> retrieval method using trypsin.
> Any help and advice gratefully rece

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