Re: Urgent:dried out specimens
|From:||Barry Rittman <email@example.com> (by way of histonet)|
Tissue will be fragile but can be treated a mummified material.
Place in Ruffers solution overnight
3 parts 96%ethanol
5 parts of 1% formalin
2 parts 5% aqueous sodium carbonate.
This renders tissue somewhat gelatinous.
decant off one third of the volume and replace with 96% ethanol. repeat
until tissue is firm.
Process to wax for soft tissue and down to water if to demineralize.
If for storing, place after the 96% ethanol, into one part glycerin plus
2 parts 70% ethanol.
"Renton, Lousie, Mrs" wrote:
> Dear Histonetters,
> I am in urgent need of a method for the resoration of dried out
> processed tissue. We had a problem with the processor over the
> weekend, and all our precious ( research ) material is all dried out
> and shrivelled.
> I recall hearing of a method that used phenol, and someone that
> mentioned fabric softener on the histonet, but I cannot find the
> literature. Bancrof has a method, but we do not have access to the
> chemicals cited.
> Is there a kind soul out there who could offer a recipe to save our
> skins? ( or in this case our bones!)
> Thank you in anticipation
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