Re: Urgent:dried out specimens

From:"" <>

Date sent:      	Mon, 23 Jul 2001 13:53:54 +0000 (GMT)
From:           	"Renton, Lousie, Mrs" <>
Subject:        	Urgent:dried out specimens

>  Dear Histonetters,
> I am in urgent need of a method for the resoration of dried out 
> processed tissue. We had a problem with the processor over the 
> weekend, and all our  precious ( research ) material is all dried out 
> and shrivelled.

Louise, commiserations, this has happened to us on a few 
occasions, almost always over the weekend, tissues left high and 
dry after leaving one of the alcohols!
The method I have always used is to place the cassettes 
immediately into a 50:50 mix of 70%alcohol and glycerol 
(assuming your tissues dried aftrer alcohol) and leave for at least 2 
hours, preferably overnight, then process from 70% alcohol the 
next day. This produces a block that you can cut but you will find 
staining looks somewhat "unusual" depending on the tissue and 
degree of drying.Used subbed or charged slides!
Good luck,
Terry Hacker,
Medical Research Council,

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