Re: Urgent:dried out specimens
Date sent: Mon, 23 Jul 2001 13:53:54 +0000 (GMT)
From: "Renton, Lousie, Mrs" <firstname.lastname@example.org>
Subject: Urgent:dried out specimens
> Dear Histonetters,
> I am in urgent need of a method for the resoration of dried out
> processed tissue. We had a problem with the processor over the
> weekend, and all our precious ( research ) material is all dried out
> and shrivelled.
Louise, commiserations, this has happened to us on a few
occasions, almost always over the weekend, tissues left high and
dry after leaving one of the alcohols!
The method I have always used is to place the cassettes
immediately into a 50:50 mix of 70%alcohol and glycerol
(assuming your tissues dried aftrer alcohol) and leave for at least 2
hours, preferably overnight, then process from 70% alcohol the
next day. This produces a block that you can cut but you will find
staining looks somewhat "unusual" depending on the tissue and
degree of drying.Used subbed or charged slides!
Medical Research Council,
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