I like! I LIKE!

Someone willing to take this button project on?

Peggy A. Wenk, HTL(ASCP)
William Beaumont Hospital
Royal Oak, MI

----- Original Message -----
From: "Morken, Tim" <tim9@cdc.gov>
To: <histonet@pathology.swmed.edu>
Sent: Thursday, July 19, 2001 12:43 PM
Subject: Thant sounds like a button motto! RE: Getting the Eosin out sol
ution


> Patsy wrote:
>
> "With HISTONET all you have to do is ask and you will receive"
>
> That could go on a Histonet button for NSH!
>
> Tim Morken
> Atlanta
>
> -----Original Message-----
> From: Patsy.Ruegg@UCHSC.edu [mailto:Patsy.Ruegg@UCHSC.edu]
> Sent: Thursday, July 19, 2001 12:22 PM
> To: histonet@pathology.swmed.edu
> Subject: Getting the Eosin out solution
>
>
>
> -----Original Message-----
> From: Ruegg Patsy
> Sent: Thursday, July 19, 2001 10:21 AM
> To: 'KOKOPELLI_2'; Ruegg Patsy
> Subject: Getting the Eosin out solution
>
>
> Thanks to all your advise.
> What I did worked like a charm.
> I put a few drops of strong (10 M) sodium hydroxide in 150 mls of DI water
> and dipped the eosin counterstained DAB reacted slides in this solution, 2
> dips was all it took and all the eosin came out without affecting the DAB
at
> all, it made the hematoxylin stained nuclei nice and blue also.
> With HISTONET all you have to do is ask and you will receive.
> Patsy Ruegg
>
> -----Original Message-----
> From: KOKOPELLI_2 [mailto:KOKOPELLI_2@email.msn.com]
> Sent: Tuesday, July 17, 2001 9:13 PM
> To: Patsy.Ruegg@UCHSC.edu
> Subject: Getting the Eosin out
>
>
> Patsy
>
> You can put the slides into some ammonia H20 like you would use to blue
your
> slides in for an H&E stain. The eosin is a acidic dye and the ammonia
being
> basic will wash out the eosin. Through some trial and error tinkering with
> stains I learned this lesson.
>
> I don't know what effect this will have on the DAB? but I would guess very
> little, since we sometimes put varied acidic & basic reagents on our
immuno
> slides for counterstaining purposes for the Docs'.
>
> If you try this and it works out, with good results please let me know. I
am
> always interested in truoble shooting techniques.
>
> Brent A. Norris H.T.(ASCP)
> Technical Supervisor
> Special Procedures Laboratory
> University of Texa