Re: Retic trouble

From:Bill Sinai <>

> Subject: Retic trouble
Dear Linda,

Over the years I have found from personal experience it is usually due to a
high concentration of ammonia left in the solution after titration.  We
usually put the solution once made and titrated to end point on the bench
for half an hour or so then check the final titration, we find we need to
add about another 1ml of silver nitrate to complete the titration.  Then
double the volume as the technique states and you should have no more
problems.  You can easily titrate the final solution if it still has an
ammonia smell when being used.
Hope this helps.

Bill Sinai
Laboratory Manager
Tissue Pathology ICPMR
P.O. Box 533
Wentworthville NSW Australia 2145

> Hi Fellow Histonetters,
> I need some help trouble shooting Modified Gomoris
> Retic and could not think of a better place to ask for
> help.  Lately, the control and/or sections are
> floating when going from the silver solution to
> distilled water and sometimes when going from
> distilled water to 20% formalin.  The sections are cut
> at 5 microns and put on Poly-L-Lysine slides.  We have
> tried drying the slides differently each time - slide
> dryer, 37 degree incubator overnight and 57 degree
> incubator for 1 hour.  We have made up all new
> reagents and the sections still float. All the
> reagents and steps done at room temp.  I do not know
> what else to try, so, any suggestions are greatly
> appreciated.
> Thanks in advance,
> Linda Stirpe
> Corning Hospital
> Corning, NY
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