Re: NitroBllue Tetrazolium

From:"Renton, Lousie, Mrs" <177louie@chiron.wits.ac.za>

Hi Dianne,

we used to use a NBT method on unfixed heart slices to determine 
whether death was caused by myocardial infarct.  Slices of tissue 
were incubated and then examined macroscopically.Infarcted 
tissue was red and normal tissue blue. I think that it "stained " for 
succinyl dehydrogenase, but I would be happy to be corrected on 
that one.
 I don't have the method to hand - don't work in that department 
anymore, but If this is the sort of thing you are looking for, I  am 
sure that I will be able to get the reference to you, should no-one 
else be able to help.

Best regards
louise
MRC
Johannesburg

On 24 Jul 01, at 14:24, Dianne Holmes wrote:

> I am trying to stain animal tissue for muscle viability.   NBT has
> been suggested.  Has anyone dealt with it and does it have to be on
> frozen tissue?   Any information about this pro

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